Oh you poor things! I'm sorry, that sounds exhausting. With Duke being so new to recovery, all I can think of is that he is in some type of pain. Behavior changes are a common pain signal. Remind us, what kind of pain medication is he on, and how often? 

When he went to sleep, was it just out of sheer exhaustion or did you do something that might have calmed him down? And what happens in the daytime when you try to do things? Is he clingy, following your around? 

Hmm ok so this is a pre-amputation behavior that's gotten worse now that he's experiencing more pain. Well, he's definitely trying to tell you that he hurts. Have you tried soft massage and palpating him gently around his body to see if he reacts to a certain area where you touch? The shoulders? Low back? 

Thanks for the refresher on his meds. He's getting the standard 3, but they may need to be fine tuned, or you can call the vet and ask for an additional pain reliever like Amantadine. That should help with his anxiety and whatever extra pain he may be experiencing, paws crossed. Give them a call and see what they think about adding it in. Also, Tramadol can cause anxiety in some dogs, we see it happen around here a lot. Was he on it before amputation? Have you ever taken it out at any point? See what your vet thinks.

Also I know I sound like a broken record about this, but rehab therapy for him will really make a difference so I hope you guys can do that (remember the Tripawds Foundation can pay for your first rehab visit .